Your mission
We are seeking a highly skilled and motivated protein biochemist who will be duelled-skilled in cell culture to join our dynamic team. This role offers an exciting opportunity to contribute to cutting-edge drug discovery projects, where the quality control of protein reagents for performing antibody immunisations, candidate screening and biophysical profiling of drug leads is critical.
What you'll do:
You will help establish and shape this new internal capability, specifically with respect to producing recombinant proteins in-house. You will have operational responsibility for an AKTA FPLC, as well as maintaining suspension/transient mammalian cell lines and/or performing relevant protein QC.
You will be agile and support drug screening and validation experiments. This could further involve performing drug discovery screening assays using recombinant proteins (e.g. ELISAs) and/or cell-based assays to determine the functional relevance of proteins (e.g. using over-expressing or primary cell lines). A willingness to be flexible and support the overall drug discovery program is key.
Molecular biology
What you'll do:
You will help establish and shape this new internal capability, specifically with respect to producing recombinant proteins in-house. You will have operational responsibility for an AKTA FPLC, as well as maintaining suspension/transient mammalian cell lines and/or performing relevant protein QC.
You will be agile and support drug screening and validation experiments. This could further involve performing drug discovery screening assays using recombinant proteins (e.g. ELISAs) and/or cell-based assays to determine the functional relevance of proteins (e.g. using over-expressing or primary cell lines). A willingness to be flexible and support the overall drug discovery program is key.
Molecular biology
- Design DNA constructs and/or vectors to overexpress proteins within recombinant systems
- Perform routine tissue culture of suspension/transient mammalian cell lines under sterile conditions, monitoring aspects such as cell counts and viability. Perform cell banking, as required
- Harvest and clarify cell culture fluid, as suitable for downstream protein purification
- Support other drug screening efforts using cell-based assays and monitoring functional readouts, such as cell viability, proliferation and cytotoxicity
- Design, execute and/or oversee experiments for the purification of recombinant proteins and antibodies.
- Develop and implement purification protocols using chromatography techniques (e.g. affinity, ion exchange and size exclusion)
- Perform experiments and/or interpret data derived from:
- QC analyses such as SDS-PAGE, Western blots, binding ELISAs, endotoxin-testing and high-pressure liquid chromatography (HPLC)biophysical analyses of proteins using techniques such as fluorescence, biolayer interferometry (BLI) and surface plasmon resonance (SPR)
- Critically analyse data describing protein stability or antibody developability (e.g. arising from thermal, physical and/or chemical stress), 3D-structure and drug-antigen interactions